Pet Allergy Testing


Allergy testing can help veterinarians and pet owners identify allergic triggers and devise a successful pet allergy treatment strategy for the patient. The ACTT® Allergy Program by ALK employs an in vitro serum IgE test for allergen identification, enabling the veterinarian to select appropriate allergens for immunotherapy treatment or avoidance.
IN VITRO SERUM IGE TESTING:

Serum IgE testing directly measures allergen-specific IgE circulating in the serum of an allergic animal. There are a number of convenient and practical advantages offered by serum IgE testing:

  • No steroid or antihistamine interference
  • Convenience of a single blood draw
  • No sedation or clipping necessary
  • Quick turn-around time
  • Food allergy testing available
  • Test results are reproducible
  • Canine, Feline, and Equine
SUPERIOR ACTT® ELISA TECHNOLOGY
  • Our affinity purified, anti-IgE antibody detects IgE only; it does not bind to IgG.
  • Test scores are not subject to misidentification or false positive due to IgG interference.
  • Our affinity chromatography process ensures that the ACTT® ELISA assay maintains both high sensitivity and high specificity for IgE, resulting in the most accurate test results.
  • Our laboratory employs rigorous quality control methods that minimize non-specific IgE binding to ensure accurate and reproducible test results for each patient.

View articles with more information on our ACTT® ELISA test in the Resources section.

Panels

If you’re looking to provide allergy relief for your dogs, cats, and horses, then you’ll want an in-depth allergy test. Successful pet allergy treatment and management begins by identifying all aspects of a pet’s allergy exposure. The ACTT® Allergy Test offers multiple testing options:

Comprehensive Panel: Our most complete allergy profile; includes the Environmental panel and Comprehensive Food.

Environmental Panel: Geographically specific panel and includes weed, tree, grass, mold, and mite allergens. This panel also includes insect and indoor allergens as well as Staphylococcus and Malassezia.

Food Panel: Food ingredients commonly found in commercial and specialty pet foods. Please call customer service for a current list of allergens tested.



AIM Preliminary Panel: detects elevated levels of IgE for 5 allergen groups (grasses, trees, weeds, molds and other). Each group will reflect a score of Positive, Borderline or Negative to indicate if further testing is necessary. CANINE ONLY.

Equine Comprehensive: Includes weed, tree, grass and mold allergens. Also included are ten feed ingredients and eleven insects, including Culicoides.

 

 

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Guidelines for Collecting a Clean Serum Sample

When possible we recommend an 8-12 hour fast prior to drawing blood. This will help avoid a lipemic sample.

Draw the blood into a red top tube.
For Equine patients we recommend drawing 15mL of blood.
For Small Animal, draw as much as is appropriate for the size of the pet.
Consider that on average we expect a 50% serum yield from the whole blood volume.

As soon as the blood has clotted, separate the clot from tube sidewalls using a wooden applicator stick.

If possible, refrigerate the clotted specimen for 4-8 hours, or overnight. This step increases clot contraction, and will increase serum yield.
Spin the tube to separate the serum from the clot.

Pour the serum off into an ALK provided tube for serum submission.
If you do not have an ALK tube you can use a serum separator or a red top tube.
Please make sure to package appropriately to protect the tube in transit.

We cannot accept whole blood for allergy testing.

If you do not have access to a centrifuge, or are concerned about the volume of serum please do not hesitate to contact our Customer Service Team. We are always happy to discuss the specific tests, serum requirements and options available to you.

Does a lipemic or hemolyzed serum sample effect test results?
Yes. IgE is a serum component, and the presence of whole blood will interfere with assay reactions. Lipemic and hemolyzed specimens require additional processing by our lab to remove lipids and residual blood cells prior to testing. This process is costly and time consuming, and can lead to delayed results for your patient. To avoid a delay in receiving your test results please check your serum specimen before you ship it to us. A rosy beige serum sample that is free of turbidity will yield the most accurate results. As always, our goal is to provide you with the most accurate test results and treatment for your patient as quickly as possible.

How To Submit a Sample

Draw Blood and Spin Down to Serum.

Transfer to ACTT vial

Fill out the order form and place the tube in the mailer

Download the free shipping label

*Remember, no steroid or antihistamine interference

 

IMPORTANT INFORMATION ABOUT SERUM COLLECTION
Does a lipemic or hemolyzed serum sample effect test results?

Yes. IgE is a serum component, and the presence of whole blood will interfere with assay reactions. Lipemic and hemolyzed specimens require additional processing by our lab to remove lipids and residual blood cells prior to testing. This process is costly and time-consuming, and can lead to delayed results for your patient.

To avoid a delay in test results, due to additional processing time, check your serum specimen before you ship it to us. A rosy beige serum sample that is free of turbidity will yield the most accurate results.

As always, our goal is to provide you with the most accurate test results and treatment for your patient as quickly as possible.

We are available by phone to answer any questions you may have when preparing a sample for testing.

Call toll free 1-800-444-2370

50% Testing Discount for Clinic Employees
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